Optimization of Extraction Process of Total Flavonoids from Rumex madaio Makino
To optimize the process of total flavonoids from Rumex madaio Makino . Basis on the results of the single factor experiments,Rumex madaio Makino was used as materials to optimize the extraction conditions of total flavonoids by using response surface methodology involved a three leves ,three variable Box-Behnken design. The optimum conditions of total flavonoids extraction were 54%ethanol as extraction solvent ,material ratio 1:99 (g/mL)and extraction time 110 min.Under the optimum conditions,the experimental total flavonoids content of Rumex madaio Makino tested was (16.7 ±0.03) mg/g,which was well matched with the predicted content. The optimum conditions can be used to extract the total flavonoids from Rumex madaio Makino.