Study on the Dissovled Rate of Selenium and Combined of Selenium in Selenium-riched Tea with Hydride Generation-atomic Fluorescence Method
The extraction of selenium and combined of selenium in selenium-riched tea were studied. Protein combined of selenium was extracted by hydrochloric acid, polysaccharide combined of selenium and nucleic combined of selenium were extracted by removing protein combined selenium with [(1∶1 phenol-methylene chloride)-isoamyl alcohol](24∶1,mL/mL). The selenium content of tea and nucleic, polysaccharide and pro-tein combined of selenium were determined by atomic fluorescence spectrometry. The results showed that:Se-protein and Se-polysaccharide accounted for about 51.65%and 25.23%of the total selenium, the most impor-tant factors influencing the dissolution rate of selenium from tea was the soaking temperature, the dunking time and frequency. The dissolution rate was 46.35%at 95℃, and soaking three minutes, the dissolution rate of selenium was 25.80%. Content of selenium could not be detected when dunked three times. The selenium ex-tracted from tea is almost organic selenium, selenium-protein>selenium-polysaccharide>selenium-nucleic. Socking tea should be three minutes and no more than two times at 95℃.
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万方数据
维普
