Study on Extraction of Total Flavonoids in Ligustrum Flowers and Its Scavenging Activity on DPPH Free Radicals
To optimize the extraction of total flavonoids in Ligustrum flowers and its scavenging activity on DPPH free radicals, the factors influencing the process parameters were determined by specotrophotometric method. The results showed the optimum conditions of the extraction were as followes:0.5 g of samples were used as the raw material, 50%ethanol solution was used as the extraction solvent with a raw material to extrac-tion solvent ratio of 1∶55 (g/mL), and the reflux extraction was carried out in 85℃water bath for 60 min. The extraction rate was 12.57% (RSD=0.74). The IC50 value of DPPH free radical scavenging rate by flavonoids in Ligustrum flowers was 11.97μg/mL, and the maximum the clearance rate of DPPH free radical was 82.06%.
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万方数据
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