Established the Vanillin-HCl Method to Determine the Procyanidins in Purple Yam
The vanillin method to determine the concentration of procyanidins in purple yam was established.The experimental conditions were optimized from five aspects:effect of illumination,reaction time,reaction temperature,the amount of vanillin and concentration of hydrochloric acid.The experimental results showed that when 1 mL extracting solution of purple yam was injected to volumetric flask,the optimal reaction conditions were as the following:5 mL of 10 g/100 mL vanillin solution,3 mL of concentrated hydrochloric acid.After diluted to 10 mL,the volumetric flask was placed in 20 ℃ for 10 minutes,and then the absorbance was detected at 500 nm.According to the results,the line of range was from 4 μg/mL to 24 μg/mL,the relative standard deviation was 1.10 ％,and the recoveries were from 98.06 ％ to 103.89 ％.The results proved that this method could be used for the determination of proanthocyanidins in purple yam.
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万方数据
维普
