Separation and Purification of Procyanidins in Grape Seed with Macroporous Adsorption Resin
To optimize the technical parameters of optimize the technical parameters of procyanidins separation and purification with macroporous resin. The vanillin-sulfuric acid spectrophotometry was used to investigate adsorption rate, adsorbtion capacity, eluting rate and purity of procyanidin. The results indicated that the opti-mum ranges of procyanidin in sample solution was 4.5 mg/mL to 6.5 mg/mL.The rate of AB-8 resin and reagent was 1:10 (g/mL),the flow rate was 1.5 BV/h and the adsorbtion time was 8 h,the adsorbtion capacity of pro-cyanidin was more than 95 %. The eluting rate of procyanidin and the purity of solid were 99 % and 94.7 %, re-spectively, by using 40 % ethyl alcohol as eluent with 6 BV of dosage. The macroporous adsorption resin method is reliable and can be applied for separation and purification of procyanidin.
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万方数据
维普
