Optimization of Extracting Technology for Total Flavonoids in Tea Tree with Response Surface Methodology
Tea Tree from Guangdong province was used as the raw materials to extract total flavonoids.A novel approach,ultrasonic-assisted method,for preparation of total flavonoids were developed.The optimal ultrasonic-assisted treatment was explored using response surface methodology. The results showed that the optimum extraction condition were ethanol concentration 60 %,liquid ratio 1:30(g/mL),extraction time 40 min.Under the optimization conditions,the experimental yield was 29.7 mg/g.The method is reasonable and feasible,can provide a theoretical reference for the development of new flavonoids in tea plant.