超声波处理对花生蛋白分子结构的影响
Effects of Ultrasound Treatment on the Structural Properties of Peanut Protein
张文 1张丽芬 1陈复生 1王培1
作者信息
- 1. 河南工业大学粮油食品学院,河南郑州450001
- 折叠
摘要
研究采用超声波技术处理花生蛋白,借助高效液相色谱、聚丙烯酰胺凝胶电泳、红外光谱等方法对超声波改性花生蛋白的结构进行了表征.结果表明,花生蛋白经超声波改性后分子量分布在279.76 kDa以上的片段增加,而低分子量(13.69 kDa~54.75 kDa)片段降低;超声波处理后花生蛋白分别在66.2 kDa附近和14.4 kDa处生成新条带;超声波改性花生蛋白的α-螺旋结构减少,β-转角结构增加.
Abstract
The structural properties of ultrasound modified peanut protein were investigated by high performance liquid chromatography(HPLC),SDS-PAGE and infrared spectroscopy(FT-IR). The results showed that ultrasound treatment resulted in an increase in molecules above a molecular weight of 279.76 kDa, while the low molecular weight fragment(13.69 kDa-54.75 kDa)decreased. After ultrasonic treatment, the peanut protein were generated at 66.2 kDa and 14.4 kDa,respectively.Meanwhile,the percentage of α-helix was reduced as well as the β-turn increased.
关键词
超声波/花生蛋白/改性/分子量/结构Key words
ultrasound/peanut protein/modified/molecular weight/structure引用本文复制引用
基金项目
河南工业大学高层次人才基金(2013BS061)
国家自然科学基金(31501535)
国家自然科学基金(31371851)
河南省教育厅自然科学项目(15A550011)
校科技创新人才(2014CXRC01)
"粮油食品加工与新技术研究"河南省优秀科技创新团队()
出版年
2018