首页|基于DNA酶表面增强拉曼的皮蛋中痕量Pb2+检测方法

基于DNA酶表面增强拉曼的皮蛋中痕量Pb2+检测方法

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表面增强拉曼散射(surface enhanced Raman scattering,SERS)光谱法常被用于重金属离子的分析与检测.将SERS与适配体和 DNA 酶相结合,能更好地对痕量重金属进行检测分析.在 pH 7.2 的三羟甲基氨基甲烷(trihydroxymethyl aminomethane,Tris)-HCl缓冲液和0.09 mol/L NaCl条件下,单链DNA(ssDNA)和DNA酶在80℃杂交成双链DNA(dsDNA).在dsDNA和混合溶液存在下,银纳米(silver nanoparticles,AgNPs)发生聚集,聚集的AgNPs与罗丹明6G(rhodamine 6G,Rh6G)作用,在613 cm-1处出现较强SERS特征峰.Pb2+可将dsDNA裂解切割,释放出ssDNA,形成比表面积和粗糙度均增大的AgNPs-ssDNA-Pb2+大分子缔合物.随着Pb2+浓度的增大,释放出的ssDNA增多,形成的AgNPs-ssDNA-Pb2+增多,AgNPs-ssDNA-Pb2+与Rh6G作用增强,导致613 cm-1处的SERS峰强度增大.据此建立DNA酶SERS法测定痕量Pb2+的新方法,在2.5×10-8 mol/L～7.5×10-7 mol/L Pb2+之间,线性关系良好,Pb2+最低测出浓度为1.0×10-8 mol/L.新方法用于检测皮蛋中的痕量Pb2+,相对标准偏差均在10 %以内,回收率在99.5 %～107.7 %之间.
A DNA Enzyme SERS Method for Determination of Trace Pb2+in Preserved Eggs
Surface enhanced Raman scattering (SERS) spectra was usually used for the analysis and detection of heavy metal ions. Combining SERS with aptamers and DNA enzyme allowed for better detection and analysis of trace heavy metals. Under the condition of pH 7.2 trihydroxymethyl aminomethane (Tris)-HCl buffer and 0.09 mol/L NaCl, single-stranded DNA(ssDNA)and DNA enzyme hybridized to distrand DNA(dsDNA)at 80℃. In the presence of dsDNA and mixed solution, the aggregated silver nanoparticles (AgNPs) interacted with rhodamine 6G(Rh6G) and exhibited a strong SERS peaks at 613 cm-1. The dsDNA was cleaved by Pb2+cleavage releasing ssDNA and forming AgNPs-ssDNA-Pb2+macromolecular association with increased surface area and roughness. With the increase of Pb2+concentration, the release of ssDNA increased, the formation of AgNPs-ssDNA-Pb2+increased, and the intensity of SERS peak at 613 cm-1 increased due to the enhanced effect of AgNPs-ssDNA-Pb2+and Rh6G. A new method for the determination of trace Pb2+by DNA enzyme SERS was established with a linear range of 2.5×10-8 mol/L-7.5×10-7 mol/L and a detection limit of 1.0×10-8 mol/L. The method was applied to the detection of trace Pb2+in preserved eggs, the relative standard deviation (RSD) was less than 10 % and the recovery was between 99.5 % and 107.7 %.

DNA enzymesilver nanoparticles (AgNPs)Pb2+rhodamine 6G (Rh6G)surface enhanced Raman scattering(SERS) spectra

庞永丰、罗杨合、吴凤莲、伍淑婕、聂辉、段振华、黎小椿

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贺州学院食品科学与工程技术研究院,广西贺州542899

大连工业大学食品学院,辽宁大连116034

DNA酶 纳米银 铅离子 罗丹明6G 表面增强拉曼光谱

国家重点研发计划广西高等学校高水平创新团队及卓越学者计划广西高校食品农产品质量安全重点实验室广西重点学科农产品加工及贮藏工程广西果蔬保鲜和深加工研究人才小高地;2019年度广西高校中青年教师基础能力提升项目贺州学院博士科研启动基金贺州学院教授科研启动基金广西特聘专家专项经费

2018YFD0901003桂教人[2018] 35号桂教科研[2014]6-97桂教科研[2013]16-1232019KY0710HZUBS201608HZUJS201613厅发[2016]21号

2019

食品研究与开发
天津市食品研究所,天津市食品工业生产力促进中心

食品研究与开发

CSTPCD北大核心
影响因子：0.561
ISSN：1005-6521
年,卷(期)：2019.40(24)
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