Anti-photoaging Effect of Ocimene on HaCaT Keratinocytes and Hs68 Fibroblasts
This study investigated the potential benefits of ocimene,an aromatic compound predominantly present in tobacco,basil essential oil,and lavender essential oil,in mitigating the effect of ultraviolet B(UVB)-induced photoaging in HaCaT keratinocytes and Hs68 fibroblasts.The photoaging-mitigating effect of ocimene in epidermal HaCaT cells was assessed by measuring oxidative stress damage markers and matrix me-talloproteinase(MMP)expression levels.The photoaging-mitigating effect of ocimene in dermal Hs68 cells was evaluated by measuring the secretion of MMP and the composition of the extracellular matrix.The results showed that in UVB-exposed epidermal HaCaT cells,ocimene effectively reduced the accumulation of reactive oxygen species and malondialdehyde but increased the activity of superoxide dismutase.The photoaging-in-duced oxidative stress was thus alleviated.Moreover,ocimene restrained the rise of UVB-induced MMP ex-pression levels.In UVB-exposed dermal Hs68 cells,ocimene exerted a protective effect on the extracellular matrix by inhibiting MMP expression and promoting collagen and hyaluronic acid secretion,indicating its pro-tective effect on skin photoaging.
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