The protein was extracted from Ganoderma lucidum spore powder by alkaline extraction and acid precipitation method,and peptides with antioxidant activity was screened out by enzymatic hydrolysis with pro-tease.By using DPPH free radical scavenging rate and degree of hydrolysis as evaluation indexes,the enzy-matic hydrolysis process of the antioxidant peptide from Ganoderma lucidum spore powder was optimized by single factor test and response surface method,and the antioxidant properties of the protease hydrolysate prod-ucts of Ganoderma lucidum spore powder and their stability under different temperatures,pH values,and metal ion conditions were investigated.The results showed that the products of Ganoderma lucidum spore pow-der protein hydrolyzed by alkaline protease showed the strongest antioxidant activity.When the enzyme addi-tion amount was 2 000 U/g,the enzymatic hydrolysis time was 3 h,the enzymatic hydrolysis temperature was 50℃,pH was 8,and the substrate concentration was 0.50%,the enzymatic hydrolysis products had the best antioxidant activity.At 60℃,the antioxidant peptide of Ganoderma lucidum spore powder showed good ther-mal stability.It was stable in acidic environment and in the presence of Mg2+.
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