Optimization of Preparation Process and Stability Analysis of Antioxidant Peptides in Ganoderma lucidum Spore Powder
The protein was extracted from Ganoderma lucidum spore powder by alkaline extraction and acid precipitation method,and peptides with antioxidant activity was screened out by enzymatic hydrolysis with pro-tease.By using DPPH free radical scavenging rate and degree of hydrolysis as evaluation indexes,the enzy-matic hydrolysis process of the antioxidant peptide from Ganoderma lucidum spore powder was optimized by single factor test and response surface method,and the antioxidant properties of the protease hydrolysate prod-ucts of Ganoderma lucidum spore powder and their stability under different temperatures,pH values,and metal ion conditions were investigated.The results showed that the products of Ganoderma lucidum spore pow-der protein hydrolyzed by alkaline protease showed the strongest antioxidant activity.When the enzyme addi-tion amount was 2 000 U/g,the enzymatic hydrolysis time was 3 h,the enzymatic hydrolysis temperature was 50℃,pH was 8,and the substrate concentration was 0.50%,the enzymatic hydrolysis products had the best antioxidant activity.At 60℃,the antioxidant peptide of Ganoderma lucidum spore powder showed good ther-mal stability.It was stable in acidic environment and in the presence of Mg2+.
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