Process Optimization and Physicochemical Characteristics Analysis of Liquid Fermentation Medium for Pholiota limonella
The fermentation medium was prepared with corn starch,soybean powder and MgSO4 as main raw materials.The medium was optimized through single factor screening,PB center combination design and re-sponse surface analysis.Under the optimized formula treatment,the fermentation medium grew to the fruited body stage,and its physical and chemical properties were analyzed by texture analyzer combined with rel-evant national standards.The results showed that when the concentration of corn starch(carbon source)was 47.396 g/L,soybean powder(nitrogen source)was 55.707 g/L,and MgSO4(inorganic salt)was 6.363 g/L,the mycelial biomass in the medium was the highest,reaching 2.094 g,and the optimization amount was in-creased by 182.98%.The hardness,elasticity and cohesiveness of the Pholiota limonella grown under these conditions were(16.18±0.37)N,(1.25±0.05)mm and(0.25±0.02)mJ,all of which were in line with rel-evant national standards.The optimized culture medium fermentation of Pholiota limonella has the characteris-tics of high antioxidant activity,high biomass and high compactness.