首页|滑子菇柠檬磷伞种液体发酵培养基工艺优化与理化特性分析

滑子菇柠檬磷伞种液体发酵培养基工艺优化与理化特性分析

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以玉米淀粉、黄豆粉、MgSO4 为主要原料制作滑子菇培养基.通过单因素筛选、PB中心组合设计及响应面分析对发酵培养基进行优化,在优化配方处理下,待其生长到子实体阶段,通过质构仪测定结合相关国家标准分析其理化性质.结果表明,当玉米淀粉(碳源)浓度为 47.396 g/L、黄豆粉(氮源)浓度为 55.707 g/L、MgSO4(无机盐)浓度为 6.363 g/L,培养基中菌丝生物量最高,达到 2.094 g,优化量提升 182.98%.此条件下生长出的滑子菇硬度为(16.18±0.37)N,弹性为(1.25±0.05)mm,凝聚性为(0.25±0.02)mJ,其各项数值均符合相关国家标准规定.采用优化后培养基发酵培育的滑子菇具有高抗氧活性、高生物量、高致密性的特点.
Process Optimization and Physicochemical Characteristics Analysis of Liquid Fermentation Medium for Pholiota limonella
The fermentation medium was prepared with corn starch,soybean powder and MgSO4 as main raw materials.The medium was optimized through single factor screening,PB center combination design and re-sponse surface analysis.Under the optimized formula treatment,the fermentation medium grew to the fruited body stage,and its physical and chemical properties were analyzed by texture analyzer combined with rel-evant national standards.The results showed that when the concentration of corn starch(carbon source)was 47.396 g/L,soybean powder(nitrogen source)was 55.707 g/L,and MgSO4(inorganic salt)was 6.363 g/L,the mycelial biomass in the medium was the highest,reaching 2.094 g,and the optimization amount was in-creased by 182.98%.The hardness,elasticity and cohesiveness of the Pholiota limonella grown under these conditions were(16.18±0.37)N,(1.25±0.05)mm and(0.25±0.02)mJ,all of which were in line with rel-evant national standards.The optimized culture medium fermentation of Pholiota limonella has the characteris-tics of high antioxidant activity,high biomass and high compactness.

Pholiota namekoPholiota limonellamedium optimizationphysicochemical characteristics analysisliquid strain

王逸初、杨秀婷、贺晓龙、高小朋

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延安大学生命科学学院,陕西 延安 716000

微生物资源开发与绿色循环利用陕西省高校工程研究中心,陕西 延安 716000

滑子菇 柠檬磷伞 培养基优化 理化特性分析 液体菌种

2025

食品研究与开发
天津市食品研究所,天津市食品工业生产力促进中心

食品研究与开发

影响因子:0.561
ISSN:1005-6521
年,卷(期):2025.46(1)