The fermentation medium was prepared with corn starch,soybean powder and MgSO4 as main raw materials.The medium was optimized through single factor screening,PB center combination design and re-sponse surface analysis.Under the optimized formula treatment,the fermentation medium grew to the fruited body stage,and its physical and chemical properties were analyzed by texture analyzer combined with rel-evant national standards.The results showed that when the concentration of corn starch(carbon source)was 47.396 g/L,soybean powder(nitrogen source)was 55.707 g/L,and MgSO4(inorganic salt)was 6.363 g/L,the mycelial biomass in the medium was the highest,reaching 2.094 g,and the optimization amount was in-creased by 182.98%.The hardness,elasticity and cohesiveness of the Pholiota limonella grown under these conditions were(16.18±0.37)N,(1.25±0.05)mm and(0.25±0.02)mJ,all of which were in line with rel-evant national standards.The optimized culture medium fermentation of Pholiota limonella has the characteris-tics of high antioxidant activity,high biomass and high compactness.
维普
万方数据
