This study developed an ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method based on multiple secondary interactions of partition,which was dominated,dipole interaction,hydrogen bonding,adsorption,and ion interaction for retention and separation to detect the resi-dues of 16 aminoglycoside veterinary drugs in pork.The samples were extracted by phosphate buffer solution(pH4.0)containing 2%trichloroacetic acid,0.04 mmoL/L ethylenediaminetetraacetic acid disodium salt(EDTA-2Na),0.05 moL/L sodium pentanesulfonate,and 0.05 moL/L sodium hexanesulfonate,purified by HLB solid-phase extraction column(6 mL/200 mg),retained and separated by an Agilent Hilic plus column(2.1 mm×100 mm,3.5µm)with the use of hydrophilic interaction,and detected by a triple quadrupole tan-dem mass spectrometer.There was a good linear correlation in the range of 50-1 500 ng/mL with correlation co-efficients≥0.993.The limit of detection was 3.8-19.7µg/kg,and the limit of quantitation was 12.5-65.8µg/kg.At the spiking levels of 50,250,500µg/kg,the average recovery was 79.6%-115.3%,and the relative stan-dard deviation(RSD)was 0.8%-14.6%.This method can simultaneously detect 16 aminoglycoside antibiotics in pork with simple pretreatment,good stability,high accuracy,and high sensitivity,which can meet the regulatory requirements for the detection of aminoglycoside residues in pork.
关键词
兽药残留/氨基糖苷类兽药/多次级相互作用/超高效液相色谱-质谱法/猪肉
Key words
veterinary drug residues/aminoglycoside veterinary drugs/multiple secondary interactions/ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)/pork
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