A rapid and accurate method was established for determining the content of ethyl carbamate in a large number of Baijiu samples based on liquid chromatography-tandem mass spectrometry.An Accucore aQ chromatographic column(150 mm×2.1 mm,2.6µm)was used for gradient elution,which was carried out with methanol and 0.1%(volume ratio)formic acid aqueous solution as mobile phases at the flow rate of 0.3 mL/min.In the positive ion mode of electrospray ionization(ESI),selected reaction monitoring(SRM)was used for de-tection.The results showed that the content of ethyl carbamate determined by the established method presented a linear relationship(R2=0.9996),and the limits of detection and quantitation were 0.114µg/L and 0.380µg/L,respectively.The recovery range was 103.06%-106.34%,and the relative standard deviation(RSD)was 1.19%-2.05%.The RSD(n=6)of ethyl carbamate determined by the established method for 5 brands of Bai-jiu purchased from the market was 1.84%-3.49%.
关键词
高效液相色谱-串联质谱法/白酒/氨基甲酸乙酯/含量/测定方法
Key words
high performance liquid chromatography-tandem mass spectrometry/Baijiu/ethly carbamate/content/determination method
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