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液相色谱-串联质谱法测定白酒中氨基甲酸乙酯含量

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为建立一种适用于检测大量样品且能快速准确地检测出白酒中氨基甲酸乙酯含量的方法,本研究采用液相色谱-串联质谱法建立白酒中氨基甲酸乙酯含量的测定方法.采用Accucore aQ液相色谱柱(150 mm×2.1 mm,2.6µm),以甲醇和 0.1%(体积比)甲酸水溶液为流动相进行液相梯度洗脱,流速为 0.3 mL/min;在电喷雾电离的正离子模式下,采用选择反应监测扫描模式进行检测.结果表明,该方法标准曲线线性良好,相关系数R2=0.999 6,检出限为0.114µg/L,定量限为 0.380µg/L,通过加标验证,回收率为 103.06%~106.34%,相对标准偏差(relative standard devia-tion,RSD)为 1.19%~2.05%.对市场购买的 5 种品牌白酒进行 6 次重复检测,相对标准偏差(RSD)为 1.84%~3.49%.
Determination of Ethyl Carbamate in Baijiu by Liquid Chromatography-Tandem Mass Spectrometry
A rapid and accurate method was established for determining the content of ethyl carbamate in a large number of Baijiu samples based on liquid chromatography-tandem mass spectrometry.An Accucore aQ chromatographic column(150 mm×2.1 mm,2.6µm)was used for gradient elution,which was carried out with methanol and 0.1%(volume ratio)formic acid aqueous solution as mobile phases at the flow rate of 0.3 mL/min.In the positive ion mode of electrospray ionization(ESI),selected reaction monitoring(SRM)was used for de-tection.The results showed that the content of ethyl carbamate determined by the established method presented a linear relationship(R2=0.9996),and the limits of detection and quantitation were 0.114µg/L and 0.380µg/L,respectively.The recovery range was 103.06%-106.34%,and the relative standard deviation(RSD)was 1.19%-2.05%.The RSD(n=6)of ethyl carbamate determined by the established method for 5 brands of Bai-jiu purchased from the market was 1.84%-3.49%.

high performance liquid chromatography-tandem mass spectrometryBaijiuethly carbamatecontentdetermination method

文永、潘成康、何开萍、陈明学、唐云容、冯小兵、韦露露、仁蓉、刘春丽、朱安然

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贵州习酒股份有限责任公司,贵州遵义 564622

高效液相色谱-串联质谱法 白酒 氨基甲酸乙酯 含量 测定方法

2025

食品研究与开发
天津市食品研究所,天津市食品工业生产力促进中心

食品研究与开发

影响因子:0.561
ISSN:1005-6521
年,卷(期):2025.46(1)