In vivo and in vitro Hypoglycemic Activity of Alcoholic Extract from Smilax glabra Roxb and Its Principal Component Astilbin
In vivo and in vitro hypoglycemic activities of Smilax glabra Roxb's(E-RSG)alcoholic extract and its main component astilbin were compared in this study.Inhibitory activity of E-RSG and astilbin against α-glucosidase and pancreatic lipase were determined,respectively.Fluorescence quenching rates of the samples on the enzymes were examined.A diabetic mouse model was established by the alloxan method to investigate the in vivo hypoglycemic activity of astilbin and E-RSG.Results showed that,compared to astilbin,E-RSG ex-hibited a significantly higher inhibitory effect against α-glucosidase and pancreatic lipase.The fluorescence quenching rate constant of the extract toward the enzymes was 3.32 and 5.89 times that of astilbin,respec-tively,indicating that E-RSG's affinity with the enzymes was stronger.Compared with the diabetic model group,both E-RSG and astilbin significantly reduced fasting blood glucose of diabetic mice(P<0.01),remark-edly increased glucose tolerance.Besides,alloxan-induced body weight loss was ameliorated,and total choles-terol and triglyceride levels were decreased.In addition,hepatic and renal indexes were significantly decreased(P<0.05),exerting a protective effect against hyperglycemia-induced hepatic and renal injuries.Compared to astilbin,E-RSG presented better physiological activity.
万方数据
维普
