Culture Condition Optimization of a Brevibacillus borstelensis Strain for Producing Exopolysaccharides and Analysis of Exopolysaccharide Emulsifying Performance
The culture conditions of Brevibacillus borstelensis S8 for producing exopolysaccharides(EPS)were explored by single-factor experiments to improve the production efficiency of EPS.The culture conditions for producing EPS were optimized by Plackett-Burman design and steepest ascent experiments.The EPS produced by strain S8 was mixed with different hydrophobic substrates to investigate its emulsifying performance,with Tween 80 as a positive control.The results showed that the optimal culture conditions of the strain for EPS pro-duction were the inoculation amount of 4.56%,carbon source addition at 30.04 g/L,and culture for 39.9 h.Un-der these conditions,the EPS yield reached(107.81±1.47)mg/L.The EPS produced by B.borstelensis S8 had good emulsifying performance on hydrophobic substrates,with the emulsifying performance better than that of Tween 80 at the time points of 12 h and 24 h.
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