摘要
建立高效液相色谱检测桦褐孔菌粗提物中原儿茶酸、原儿茶醛和紫萁酮 3 种化合物含量的方法,对比评价超声辅助提取法、回流提取法、微波辅助提取法、酶解提取法 4 种不同提取方法对桦褐孔菌粗提物中活性成分含量的影响.结果表明,该检测方法精密度较高,3 种化合物的精密度的相对标准偏差(relative standard deviation,RSD)分别为 0.14%、0.06%、0.20%;重复性 RSD 分别为 1.67%、1.98%、2.22%;在 24h 内稳定性较好,其 RSD 分别为0.20%、0.61%、0.25%.对比不同提取方法对 3 种化合物的影响,发现采用超声辅助提取法得到的目标化合物产量较高,适用于桦褐孔菌中主要活性成分的提取.该研究建立的HPLC法检测桦褐孔菌粗提物中原儿茶酸、原儿茶醛和紫萁酮含量的结果准确.
Abstract
A high performance liquid chromatography(HPLC)method was established to determine the con-tent of protocatechuic acid,protocatechuic aldehyde,and osmundacetone in the crude extract of Inonotus obliquus).The effects of four different extraction methods,i.e.,ultrasonic extraction,reflux extraction,microwave-assisted extraction,and enzyme-assisted extraction,on the content of active ingredients in the crude extract of I.obliquus were compared and evaluated.The results indicated that the assay had high precision,with relative standard deviations(RSD)of 0.14%,0.06%,and 0.20%for the three compounds.It also showed good reproducibility,with RSD values of 1.67%,1.98%,and 2.22%,respectively,and good stability over 24 h,with RSD values of 0.20%,0.61%,and 0.25%,respectively.A comparison of the effects of different extraction methods on the three compounds revealed that the target compounds obtained by ultrasound-assisted extraction gave higher yields and were suitable for the extraction of the main active components in I.obliquus.The HPLC method established in this study for determining the content of protocatechuic acid,protocatechuic aldehyde,and osmundacetone in the crude extract of I.obliquus produced accurate results.
维普
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