Effects of nitrogen addition and moisture change on microbial diversity and community composition in subtropical plantation red soil
The intensification of nitrogen deposition and the change of global rainfall pattern are important features of global change.The input of exogenous nitrogen and the change of soil water conditions will inevitably affect soil microbial diversity and community composition.Illumina Miseq high-throughput sequencing technology was used to study the effects of nitrogen addition,water change and their interaction on bacterial and fungal diversity and community composition of red soil in a modeling subtropical plantation,as well as their relationships with soil properties.The results showed that:soil bacterial diversity decreased with the decrease of water content,and the inhibition effect was enhanced by the addition of nitrogen.The Shannon index of soil fungi under medium water treatment was significantly higher than that under low water treatment.Nitrogen addition significantly decreased the relative abundance of Acidobacteria,while increased the relative abundance of Actinobacteria and Bacteroidetes.In the soil,the relative abundance of Acidobacteria and Bacteroidetes increased with the increase of soil water content,while Actinobacteria and Firmicutes were on the contrary.Drought intensified the inhibition effect of nitrogen addition on the relative abundance of Acidobacteria.Nitrogen addition and soil water interaction significantly affected the relative abundance of Cyanobacteria.But nitrogen addition,soil moisture and their interactions had no significant effects on soil fungal community composition.The results of redundancy analysis showed that the changes of soil bacterial community composition were significantly related to soil water content,pH,NO3--N content and TN,which accounted for 65.6%,26.1%,21.5%and 20.7%of the changes of soil bacterial community composition,respectively.These results suggest that nitrogen addition may alter the intensity and direction of the effects of drought on soil bacterial diversity and community composition.
nitrogen depositionsoil water contentmicrobial diversitymicrobial community composition
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