Integrated biomarker response index to assess decabromodiphenyl ether induced oxidative stress in liver of Lutjanus argentimaculatus
Lutjanus argentimaculatus were exposed to different concentrations of decabromodiphenyl ether(BDE209);antioxidant enzyme activities and drug metabolic enzyme contents were analyzed,and integrated biomarker responses index(IBR)was used to assess the integrated response of biomarkers in liver.The results showed that activities of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD)and glutathione S-transferase(GST)increased in liver when fish exposed to BDE209 for 3 and 7 days,while no dose effect was detected.These enzyme activities of all experimental groups had no significant difference from that of the control group at day 30.Compared with the control group,cytochrome P450(P450)content showed a trend of decrease at the initial period,and then increased significantly.On the contrary,cytochrome b5(b5)content increased and then decreased with exposure time extension.These results showed oxidative stress effects in fish liver caused by BDE209 exposure.The highest IBR index was observed at day 7 compared to other sampling times.IBR of all challenged groups was higher than the control group,and increased gradually as BDE209 concentration increased.Compared to other biomarkers,POD activity,P450 and b5 content could be used as potential biomarkers of BDE209 stress.According to the general trend of IBR index,IBR was suitable to assess the effects of environmental concertation of BDE209 on L.argentimaculatus in an integrated approach,and was of great significance for the comprehensive evaluation of the ecological toxicological effects of BDE209.
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维普
