Effects of crop rotation on soil bacterial community structure in multi-year continuous cropping cotton fields
Xinjiang cotton fields have a serious of problems on continuous cropping;crop rotation is an effective measure to alleviate the obstacle.High-throughput sequencing was carried out on the soil of alfalfa,soybean,peanut,tomato,corn and sugar beet in rotation continuous cropping cotton fields,and effects of six crops on the soil microecology in cotton fields were evaluated in terms of the changes of soil bacterial community structure,screening crops that could significantly improve the soil microecology in continuous cropping cotton fields.The results showed that the bacterial community structure in the rhizosphere soil of soybean,peanut and alfalfa was similar to that of cotton;the ACE index and Chao1 index of soil bacteria in rhizosphere soil of soybean,peanut and alfalfa were increased but there was no significant difference with cotton.While tomato,corn and sugar beet had a greater effect on soil bacterial community structure in field,the bacterial population number and ALPHA diversity in the rhizosphere soil of tomato,corn and sugar beet were significantly lower than those of cotton.In terms of species composition,Proteobacteria,Actinobacteria and Acidobacteria were dominant phyla in soil.Among them,the abundance of Proteobacteria in tomato rhizosphere soil was the highest(58.4%),and that in alfalfa rhizosphere was the lowest(40.5%).The abundance of Actinomycetes in alfalfa rhizosphere was the highest(15.8%)and in tomato rhizosphere was the lowest(6.5%).Soybean rhizosphere had the highest abundance of Acidobacteria which was 10.2%,and tomato rhizosphere had the lowest at 5.5%.The significantly different bacterial populations were mainly concentrated in the rhizosphere of alfalfa and soybean,but the short-term rotation had no significant effect on the relative abundance of the main beneficial bacterial populations in the soil.In conclusion,short-term rotation of soybean,peanut and alfalfa had little effect on soil bacterial community structure in cotton field.However,tomato,corn and sugar beet could significantly reduce soil bacterial diversity and population number in cotton field,among which sugar beet had the greatest effect.
cotton field in Xinjiangcrop rotationbacterial community diversitysoil microecologyleguminous crop
万方数据
维普
