Molecular mechanisms of suberinization in the roots of Bruguiera gymnorrhiza subjected to salt stress
RNA-Seq(RNA sequencing)and qRT-PCR(Quantitative Real-time PCR)were utilized to elucidate the molecular mechanisms involved in suberin biosynthesis in the roots of Bruguiear gymnorrhiza in response to salt stress.A total of 36561 DEGs(differentially expressed genes)were identified,including 36351 upregulated genes and 210 downregulated genes.GO(Gene Ontology)annotation showed that these DEGs were primarily associated with cell nitrogen compound metabolism,cell and cell composition,and structural molecule activity.KEGG(Kyoto Encyclopedia of Genes and Genomes)metabolic pathway annotation indicated that the differentially expressed genes were significantly enriched in ribosomes and oxidative phosphorylation.We also observed significant enrichment in tropane,piperidine,and polyketide alkaloid biosynthesis pathways.Furthermore,39 genes related to suberinization,such as monomer synthesis,transmembrane transport and polymerization,were also identified.Nevertheless,qRT-PCR results also showed that long chain acyl-CoA synthetase 1(LACS1),glutathione S-transferase(GST)and the Myb superfamily(MYB)were upregulated under salt stress.This study provides fundamental insights into the mechanism of salt tolerance associated with suberinization and may have significant implications for mangrove protection and restoration.
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