生态科学2024,Vol.43Issue(5) :112-120.DOI:10.14108/j.cnki.1008-8873.2024.05.013

人体排泄物的除臭复合微生物菌剂的构建与效果分析

Construction and effect analysis of compound microbial agent for deodorizing human feces

张婷玥 辛伟明 李金玲 刘旭亮 郭光耀 邱忠平 杨柳 刘艳秋
生态科学2024,Vol.43Issue(5) :112-120.DOI:10.14108/j.cnki.1008-8873.2024.05.013

人体排泄物的除臭复合微生物菌剂的构建与效果分析

Construction and effect analysis of compound microbial agent for deodorizing human feces

张婷玥 1辛伟明 1李金玲 1刘旭亮 1郭光耀 2邱忠平 1杨柳 3刘艳秋1
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作者信息

  • 1. 西南交通大学生命科学与工程学院,四川省天然药物仿生合成工程研究中心,成都 610031
  • 2. 西南交通大学材料科学与工程学院,成都 610031
  • 3. 四川炬原玄武岩纤维科技有限公司,达州 635755
  • 折叠

摘要

为解决厕所异味、难闻等问题,从各除臭菌种中筛选出 4 种除臭微生物,针对单个菌株及复合菌剂对人体排泄物中主要气味物质 H2S 和 NH3 的降解作用展开研究,使用亚甲基蓝比色法和纳氏试剂比色法测定排泄物中 H2S 和NH3 含量,旨在构建高效除臭复合菌剂.单因素实验结果表明,4 种菌株中,细黄链霉菌(Streptomyces microflavus)和产朊假丝酵母(Candida utilis)对H2S的去除能力较强,去除率均高于 50%;枯草芽孢杆菌(Bacillus subtilis)和植物乳杆菌(Lactobacillus Plantarum)对NH3 的去除能力较强,去除率分别为 28.93%和 26.10%.正交实验结果显示,正交 2 实验组复合菌剂对 H2S的去除率达到 72.10%,正交 7 实验组复合菌剂对 NH3 的去除率达到 44.99%.经 SPSS软件数据分析得出,去除 H2S 的复合微生物菌剂的最优配比为 V(产朊假丝酵母):V(细黄链霉菌):V(植物乳杆菌):V(枯草芽孢杆菌)=2:1:3:2,去除 NH3 的复合微生物菌剂的最优配比为 V(产朊假丝酵母):V(细黄链霉菌):V(植物乳杆菌):V(枯草芽孢杆菌)=1:1:3:2.

Abstract

To solve the problems of toilet odor,four types of deodorizing microorganisms from different deodorizing strains were screened,and the degradation of hydrogen sulfide(H2S)and ammonia(NH3)in human feces were studied by single and compound microbial agent,aiming to develop a highly efficient deodorizing compound microbial agent.The composition of H2S and NH3 in excreta was determined by Methylene Blue colorimetry and Nessler colorimetry.The results showed that among the four strains,Streptomyces microflavus and Candida utilis had the most significant H2S removal ability with the removal rates both higher than 50%.Bacillus subtilis and Lactobacillus Plantarum showed strong NH3 removal ability,with removal rates of 28.93%and 26.10%,respectively.The compound microbial agent removal ability of H2S and NH3 was further analyzed by orthogonal experiment.The removal rate of H2S in test group 2 reached as high as 72.10%.The removal rate of NH3 in test group 7 reached 44.99%.Finally,data analysis by SPSS software showed that the optimal volume ratio of compound microbial agent to remove H2S was V(Candida utilis):V(Streptomyces microflavus):V(Lactobacillus Plantarum):V(Bacillus subtilis)=2:1:3:2.The optimal volume ratio of compound microbial agent to remove NH3 was V(Candida utilis):V(Streptomyces microflavus):V(Lactobacillus Plantarum):V(Bacillus subtilis)=1:1:3:2.

关键词

人体排泄物/除臭微生物/复合菌剂/硫化氢/氨气

Key words

human feces/deodorant microorganism/compound microbial agent/hydrogen sulfide/ammonia

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基金项目

中央高校基本科研业务费专项资金项目(2682024ZTPY015)

中央高校基本科研业务费专项资金项目(2682022ZTPY045)

四川省达州市政府专项资金项目(2019H010791)

国家自然科学基金项目(51978576)

出版年

2024
生态科学
广东省生态学会 暨南大学

生态科学

CSTPCDCSCD北大核心
影响因子:0.464
ISSN:1008-8873
参考文献量34
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