Effects of Cunninghamia lanceolata plantations at different developmental stages on soil microbial community structure.
By the method of denaturing gradient gel electrophoresis(DGGE),the polymorphism of soil bacterial 16S rRNA and fungal 28S rRNA specific fragments in 3-,11-,17-,21-,and 24-year old Cunninghamia lanceolata plantations was,studied,with the effects of C. lanceolata plantation's development stage on soil microbial community structure analyzed.The Shannon diversity index(H) and richness(S)showed that the soil microbial community diversity increased significandy with the development of C.lanceolata plantation,but was still significantly lower than that in secondary broad-leaved forests(P<0.05).Cluster analysis(UPGMA)indicated that the soil fungal community similarity in difierent aged C.lanceolata plantations was less than 60%,whereas that of soil bacterial community similarity Was up to 65%,suggesting that the shift of soil fungal community structure with the development of C.lanceolata plantation was more obvious than that of soil bacterial community structure.The soil microbial diversity had significant correlations with soil available N content and C/N ratio(P<0.05).This study indicated that longterm silviculture with pure plantations could affect soil microbial community via altering soil properties,which in turn,could affect the nutrient cycling in forest ecosystem and the plantation productivity.
Cunninghamia laneeolata plantationsoil microbial community structure16S rDNA28S rDNAPCR-DGGE
国家科技期刊平台
NSTL
万方数据
维普
