Effects of copper stress on the growth and genomic DNA methylation of Arabidopsis thaliana seedlings
In this study,the changes of DNA methylation levels and patterns in Arabidopsis thaliana seedlings under 0-3.0 mg · L-1 Cu2+ stress were studied using methylation-sensitive amplified polymorphism (MSAP) technique,and the sensitivity of the fresh weight and root growth of seedlings to Cu stress were compared.The results showed that:(1) Under Cu stress (0.25,1.0 mg · L-1) for 15 days,no significant changes were observed in root growth and fresh weight of A.thaliana seedlings compared with the control group,but the genomic MSAP rate first increased and then decreased with the increase of Cu2+ concentration.The MSAP rates were 15.93％,16.28％ and 15.83％ at 0,0.25,1.0 mg · L-1 Cu2+,respectively;(2) At the high concentration of Cu2+(3.0 mg · L-1),the root length was shortened,the fresh weight was significantly decreased and the MSAP rate was reduced to 14.26％;(3) Under Cu stress (0.25-3.0 mg · L-1),the number of methylation (M) type and demethylation (D) type sites increased significantly,and Msp Ⅰ was more sensitive to stress response compared to Hpa Ⅱ.Therefore,the MSAP of A.thaliana was sensitive to low concentration Cu2+ stress,which could be used for early diagnosis and ecological risk assessment of Cu pollution.
国家科技期刊平台
NSTL
万方数据
维普
