Effects of mixed Fraxinus mandshurica and Larix olgensis plantation on the function diversity of soil microbial community
In order to evaluate the effect of afforestation mode on soil physical and chemical properties and the function diversity of microbial community,we studied soil pH,nutrient content and metabolism function diversity of soil microorganisms in three plantation forests,including pure coniferous forest (Larix olgensis),pure broadleaf ash forest (Fraxinus mandshurica),and their mixed forest (F.mandshurica + L.olgensis) in Douling,Xinbin of Liaoning Province.The results showed that,compared to the coniferous forest,the concentrations of total N,available N,total P and available P in the mixed forest increased by 37.1％,35.6％,9.5％ and 12.9％,respectively.Total carbon source utilization capacity (AWCD) by soil microbial communities was in the order of F.mandshurica forest ＞ mixed forest ＞ L.olgensis forest,indicating that soil microbial metabolic activity in L.olgensis forest was the lowest.There were differences in microbial utilization rate of six major carbon sources among the three forests.The mixed forest had significantly higher microbial utilization rates in L-arginine,L-asparagine,pyruvic acid methyl ester,putrescine,N-acetyl-D-glucosamine,D-galacturonic acid,D-glucosaminic acid,and 4-hydroxybenzoic acid (eight kinds of carbon source) than the coniferous forest.Principal component analysis showed that carbohydrate and amino acids were important factors affecting soil metabolic activity.There was no significant difference in Simpson's diversity index of soil microbes among the three forests.The Shannon's index was in order of F.mandshurica forest ＞ mixed forest ＞ L.olgensis forest.Our results demonstrated that planting F.mandshurica and L.olgensis trees together can improve soil fertility and promote soil microbial activity.
microbial functional diversitycarbon utilizationsoil physical and chemical propertiesprincipal component analysisBiolog microplate
国家科技期刊平台
NSTL
万方数据
维普
