Wood-degrading enzymes and genetic diversity of Piptoporus betulinus in Northeast China
Piptoporus betulinus is a parasitic fungus on Betula spp.,and catalyzes wood fiber into carbohydrate using its secretory enzymes.However,wood-degrading enzymes by this brown-rot fungus and its genetic diversity have been poorly studied.Here we collected fruiting bodies of P.betulinus from four sites in Northeastern China,detected their cellulase and hemicellulase activities,and then analyzed genetic diversity using target region amplified polymorphism (TRAP) marker.The results showed that enzymatic activities of strains collected from Maoershan and Dunhua were relatively higher than that from the other sites,and variance analysis result indicated that there existed significant differences between different sites and different treated days.All samples were divided into two groups by multidimensional scaling and heat map analysis,which basically coincided well with the pattern of geographic distance similarity,and found that a significant difference in enzyme production existed among different strains.In addition,based on analysis of molecular variance,genetic differentiation mainly occurred within population.These findings provide a technical support for rapidly developing enzyme system and engineering fungi,and lay a foundation for wood protection and the utilization of economic fungi in future.
国家科技期刊平台
NSTL
万方数据
维普
