Oxidative response of earthworm Eisenia fetida to Cd2+ stress based on factor analysis
When earthworms are exposed to pollutants,their antioxidant system will have responses immediately.Consequently earthworms are widely used to monitor various pollutants as sensitive bio-indicator organisms.However,there are a large number of indices associated with the oxidative stress response.Finding out the key monitoring indices in the stress process becomes a practical demand of pollution monitoring and warning process.Factor analysis approach is a statistical method that uses a few factors to replace many original factors.This paper is aimed at analyzing and sorting factors related to Cd2+ on the earthworm Eiseniafetida under oxidative stress.Our experiment was divided into two groups:short-term test and long-term test.The former one was for ten days,taking out an earthworm every day for analysis;The latter test lasted for 30 days,taking out an earthworm every ten days.The Cd2+ concentration was set at 0,50,100,125,250 and 500 mg · kg-1.Post-clitellum segments of earthworms were chosen to determine SOD,POD,GPX,GST,CAT,VE,MDA and ACHE.The results showed that in the short-term group,the main bioindicator associated with oxidative stress reaction was CAT at the exposure time of 1-3 days,MDA at 4-5 days,POD at 6-7 days,and GST and GPX at 8th day,CAT at days 9-10.With the long-term test,GPX was the main bioindicator associated with oxidative stress reaction.
国家科技期刊平台
NSTL
万方数据
维普
