Changes of physiological metabolism and enzyme activities of Crassostrea sikamea under long-term starva-tion
To clarify the physiological and metabolic responses of Crassostrea sikamea under long-term starvation stress,we measured oxygen consumption rate,ammonia excretion rate and activities of lactate dehydrogenase,suc-cinate dehydrogenase,pyruvate kinase,superoxide dismutase,alkaline phosphatase and lysozyme in hepatopancre-as after long-term starvation(0-80 d)and refeeding(90-118 d).The results showed that oxygen consumption and ammonia excretion rate of C.sikamea decreased significantly under long-term starvation(P＜0.01).With the pro-longation of starvation,the activities of lactate dehydrogenase,succinate dehydrogenase,pyruvate kinase,superox-ide dismutase,and alkaline phosphatase in C.sikamea showed a trend of fluctuating decline,while lysozyme activi-ty showed a trend of fluctuating rise.After refeeding,oxygen consumption and ammonia excretion rate of C.sikamea significantly increased(P＜0.01).Long-term starvation may damage the metabolism and immune functions of C.sikamea,and C.sikamea,and they respond to long-term starvation stress by reducing metabolic rate and metabolic enzyme activities.Our results provide a reference for the study of metabolic regulation mechanism of marine shellfish under starvation stress.
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维普
