首页|人工修复草地土壤丛枝菌根真菌群落组成对不同放牧年限的响应

人工修复草地土壤丛枝菌根真菌群落组成对不同放牧年限的响应

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为探究人工修复草地不同放牧年限对土壤丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)与植物共生关系及群落组成的影响,本研究调查了云南省种羊繁育推广中心撂荒地、放牧25年和放牧5年草地植物群落多样性、菌根侵染率、土壤AMF孢子密度以及菌丝密度,应用Illumina测序分析了土壤AMF的群落组成.结果表明,相比撂荒地,放牧增加了草地中豆科植物比例,降低了 AMF菌丝密度,但放牧及撂荒地土壤AMF孢子密度和菌根侵染率没有显著差异.与撂荒地相比,放牧增加了 AMF群落丰富度,但随着放牧时间的增加,AMF群落丰富度变化不显著.不同放牧年限AMF群落结构差异显著,放牧25年处理中出现了AMF特有种Glomus-group-B-Glomus-ORVIN-GLO4-VTX00278.豆科植物重要值、土壤pH、铵态氮和速效磷含量是影响AMF群落组成的主要因素.
Responses of soil AMF community composition in artificially reseeding grassland to different grazing dura-tions
To understand the effects of different grazing durations in artificial reseeding grasslands on the symbiotic relationship between soil arbuscular mycorrhizal fungi(AMF)and plants and the composition of AMF community,we investigated plant community diversity,mycorrhizal colonization rate,AMF spore density and mycelium density in soil of abandoned land,grassland grazed for 25 years,and grassland grazed for 5 years in Yunnan Provincial Breeding and Promotion Center,and analyzed the community composition of AMF in soil by Illumina sequencing.The results showed that compared with abandoned land,grazing increased the proportion of legumes in grasslands and decreased AMF mycelium density,but there was no significant difference in soil AMF spore density and mycor-rhizal colonization rate between grazed grasslands and abandoned land.Grazing increased AMF community richness compared to abandoned land,but the richness did not change significantly with increasing grazing duration.The AMF community structure varied significantly across different grazing durations.Endemic species of AMF Glomus-group-B-Glomus-ORVIN-GLO4-VTX00278 emerged in the grassland grazed for 25 years.Legume importance value,soil pH,NH4+-N and available phosphorus content were the main factors affecting the composition of AMF community.

grazingsymbiotic relationshipIllumina sequencingarbuscular mycorrhizal fungicommunity struc-ture

苏颖颖、李旭旭、李香君、弓晋超、王文、马骢毓、孙飞达、张新全、周冀琼

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四川农业大学草业科技学院,成都 611130

云南省种羊繁育推广中心,云南寻甸 655200

放牧 共生关系 Illumina测序 丛枝菌根真菌 群落结构

国家自然科学基金面上项目中国博士后科学基金四川省科技厅应用基础项目

322717762022M7122892022NSFSC1769

2023

生态学杂志
中国生态学学会

生态学杂志

CSTPCDCSCD北大核心
影响因子:1.439
ISSN:1000-4890
年,卷(期):2023.42(11)
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