Otolith strontium marking of juvenile Liza haematocheila
To clarify the safety and effectiveness of otolithic strontium markers in juvenile Liza haematocheila,oto-lith marking was carried out through soaking in different concentrations(20-100 mg·L-1)of strontium chloride hexahydrate(SrCl2·6H2O)for 1,2,and 3 d.After that the marking juvenile fish were transferred to natural sea-water for 90 d of continuing rearing.The results showed no significant difference in Sr/Ca ratio between the all marking groups and the control groups.After these fish were reared for 90 d,there was no significant difference in Sr/Ca ratio between the marked ST1-20 marking groups and control groups,while the Sr/Ca ratio of the other marking groups in area 1.1-1.3 mm away from the otolith core was significantly higher than that of the control groups.After being reared for 90 d,the effect of the Sr-marking was still visible.During the experiment period,there were no significant differences in body length,body weight,fullness and death rate between the marked groups and the control groups.For the groups,after 40 mg·L-1 Sr-marking for 1 d and 20 mg·L-1 Sr-marking for 2d,the muscle strontium content in the two marked groups gradually returned to the control level at 36 and 38 d.Our results suggested that the otolith Sr-marking method for juvenile Liza haematocheila could be effective,stable,and safe.The better marker groups are ST1-40 and ST2-20.
Liza haematocheilastrontiumotolithmark effectproliferation and release
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