Physicochemical properties and fungal community diversity in rhizosphere soil of transgenic glyphosate-resistant Brassica napus
In the study of environmental safety assessment of transgenic crops,whether the introduction of exoge-nous genes will affect the ecological balance of soil microbial populations and non-target organisms has been contro-versial.In this study,we analyzed the differences in rhizosphere soil physicochemical properties of glyphosate-resistant Brassica napus overexpressing mEPSPS(JHX6)(GMO)and the control material(J9707)(GMO-free)in different developmental stages.The fungal communities of rhizosphere soil in field and pot experiments were ana-lyzed using ITS high-throughput sequencing technology of IonS5TMXL.Results showed that there was no significant difference in physicochemical properties of rhizosphere soil between the two materials at the same developmental sta-ges.The main fungal phyla enriched in the rhizosphere soil of B.napus were Olpidiomycota and Ascomycota.There were no significant differences of alpha diversity and structure of fungal community in the rhizosphere soil of both GMO and GMO-free at the same developmental stages.There was a significant difference in the relative abundance of Olpidiomycota in the rhizosphere soil of the two materials in both pot and field experiments.Our results suggest that the mEPSPS introduction in B.napus did not affect rhizosphere soil physicochemical properties and fungal com-munity structure and diversity,but would affect the relative abundance of specific fungal species.
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