We compared the difference of metabolite components of wild ancient tea(Camellia talinensis)along an altitudinal gradient(2050,2200,2350,2500 m)of Qianjiazhai Reserve in Yunnan Province by UPLC-MS/MS,to explore the physiological and metabolic response and adaptation at different altitudes.The results showed that a total of 12 categories of metabolites and 1013 metabolites in C.talinensis were detected on the four altitudes.Fla-vonoids were the most common(23.10％),followed by phenolic acids(16.68％).A total of 430 differential metabolites were selected,with 75 shared differential metabolites.More flavonoids were enriched at 2050 m alti-tude,and more amino acids and their derivatives,nucleotides and their derivatives,alkaloids and lipid metabolites at 2200 m altitude.Phenolic acids and organic acids increased significantly at altitudes of 2350 m and 2500 m.In addition,different metabolites were significantly enriched in the flavonoid biosynthesis pathway,caffeine metabo-lism pathway and amino acid metabolism pathway at different altitudes.With increasing altitude,key metabolites of flavonoid pathway showed a down-up-down trend,while the key metabolites of caffeine and amino acid pathway showed a first increasing and then decreasing trend.
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