外源精氨酸对盐碱胁迫下绿豆苗期生长的调控效用
Regulation effect of exogenous arginine on mung bean seedling growth under salt-alkali stress
王晗昕 1王庆燕 2刘繁超 1庞园园 1方淑梅 2梁喜龙2
作者信息
- 1. 黑龙江八一农垦大学,黑龙江大庆 163319
- 2. 黑龙江八一农垦大学,黑龙江大庆 163319;黑龙江省植物生长调节剂工程技术研究中心,黑龙江大庆 163319
- 折叠
摘要
为明确外源精氨酸(Arg)对盐碱胁迫下绿豆苗期生长的调控效用,本研究以"绿丰2号"和"绿丰5号"两个绿豆品种为试验材料,以150 mmol·L-1盐碱进行胁迫处理,研究叶面喷施不同浓度Arg对绿豆苗期形态和生理指标的影响.结果表明:叶面喷施适宜浓度的Arg(1.0～1.5 mmol·L-1)可显著提高绿豆幼苗对盐碱胁迫的抗逆性,缓解植株地上部和根系生长所受损伤;光合色素含量、抗氧化酶活性(SOD、POD、CAT)和渗透调节物质含量(可溶性糖、可溶性蛋白、脯氨酸)显著上升;ROS代谢产物超氧阴离子(O2·-·)产生速率和膜脂过氧化产物丙二醛(MDA)含量显著下降;Arg通过提高绿豆苗期抗氧化酶活性及渗透调节物质含量,降低幼苗膜脂过氧化物的生成量,来保护细胞结构的完整,促进幼苗生长,从而增强绿豆苗期耐盐能力.
Abstract
To clarify the regulation effect of exogenous arginine(Arg)on the growth of mung bean seedlings under salt-alkali stress,we analyzed the effects of foliar spraying of different concentrations of Arg on morphological and physiological indices of seedlings of two mung bean cultivars,"Lyufeng 2"and"Lyufeng 5",which were treated with 150 mmol·L-1 salt-alkali.The results showed that appropriate Arg concentration(1.0-1.5 mmol·L-1)sig-nificantly improved the resistance of mung bean seedlings under salt-alkali stress,and alleviated the damage to aboveground tissue and roots.The content of photosynthetic pigments,the activities of antioxidant enzyme(SOD,POD,CAT),and osmoregulatory substances(soluble sugars,soluble proteins,proline)significantly increased,while the production rate of ROS metabolite superoxide anion(O2)and the content of membrane lipid peroxida-tion product malondialdehyde(MDA)significantly decreased.In conclusion,Arg can protect the integrity of cell structure and promote seedling growth by increasing the activities of antioxidant enzymes and osmoregulatory sub-stances and decreasing the production of membrane lipid peroxides,thus enhancing salt tolerance of mung bean seedlings.
关键词
盐碱胁迫/精氨酸/绿豆/渗透调节/保护性酶/膜脂过氧化物Key words
saline-alkali stress/arginine/mung bean/osmoregulation/protective enzyme/membrane lipid peroxide引用本文复制引用
基金项目
黑龙江省农垦总局科技攻关计划(HNK135-02-10)
国家自然科学基金(32272225)
黑龙江省自然科学基金(LH2023C079)
出版年
2024
国家科技期刊平台
NSTL
万方数据