肠道类器官在肠疾病机制研究中的运用
Application of Intestinal Organoids in The Study of Intestinal Disease Mechanism
强龙征 1毛海光 1王梦婷 1齐莉莉 1王进波1
作者信息
- 1. 浙大宁波理工学院,生物与化学工程学院,宁波 315100
- 折叠
摘要
肠道类器官由来自肠道的隐窝或干细胞在培养基质的三维(3D)支撑下构建形成,含有肠道的所有成熟细胞,已经成为研究肠道疾病机制全新且高效的平台.相较于二维(2D)细胞培养,肠道类器官不仅可以更加有效地模拟肠道的生理结构与功能,还可以在不同体外环境下更好地还原肠道的真实生态,因此在不同肠道疾病的发病机制研究中应用更为广泛.本文介绍了肠道类器官培养方式的新进展,综述了近年来肠道类器官在炎症性肠道疾病、结肠直肠癌和乳糜泻发病机制研究中的运用及进展,同时讨论了肠道类器官在药物研发与筛选方面的应用.
Abstract
Intestinal organoids are constructed by crypts or stem cells from the intestine under the 3D support of the culture matrix.They contain all mature cells of the intestine,and have become a new and efficient platform for studying the mechanism of intestinal diseases.Compared with 2D cell culture,organoids can not only more effectively simulate the physiological structure and function of the intestine,but also better restore the true ecology of the intestine in different external environments.Therefore,it is more widely used in the study of pathogenesis of different intestinal diseases.This article reviewed the new progress of intestinal organoids culture,and the application and progress of intestinal organoids in the pathogenesis of inflammatory bowel diseases,colorectal cancer and celiac disease in recent years,and also discussed the application of intestinal organoids in drug research and development and screening.
关键词
肠道类器官/肠道疾病/病理机制/药物研发Key words
intestinal organoids/intestinal diseases/pathological mechanism/drug research and development引用本文复制引用
基金项目
国家自然科学基金(31272461)
宁波市科技局(202003N4305)
宁波市科技局(2018B10095)
宁波市科技局(2017C110017)
宁波市科技局(2021Z112)
浙大宁波理工学院人才引进基金(20211018Z0216)
出版年
2024
NETL
NSTL
万方数据