miR-375 Attenuates The Migration and Invasion of Osteosarcoma Cells by Targeting MMP13
Objective To explore whether miR-375 regulates the malignant characteristics of osteosarcoma(OS)by influencing the expression of MMP13.Methods Plasmid DNAs and miRNAs were transfected into OS cells and HEK293 cells using Lipofectamine 3000 reagent.Real-time quantitative polymerase chain reaction was performed to measure the expression of miR-375 and MMP13 in OS patients and OS cells.Western blot was performed to analyze the MMP13 protein in the patients with OS and OS cells.The targeting relationship between miR-375 and MMP13 was analyzed by luciferase assay.Migration and invasion were analysed by heal wound and transwell assays,respectively.Results miR-375 expression in OS tissues was lower than that in normal tissues.The expression of MMP13 was upregulated in OS tissues.MMP13 expression was negatively correlated with miR-375 expression in patients with OS.Migration and invasion were significantly inhibited in OS cells with the miR-375 mimic compared with OS cells with the miRNA control.MMP13 partially reversed the inhibition of migration and invasion induced by miR-375 in the OS cells.Conclusion miR-375 attenuates migration and invasion by downregulating the expression of MMP13 in OS cells.
万方数据
维普
