Connectomics,a research field in neuroscience studying the synaptic connectivity patterns between neurons across different brain regions,is crucial for understanding neural computations underlying complex functions such as emotion,learning,and cognition.Specifically,micrometer-resolution mesoscale connectomics has become the most widely used technology in rodent neuroscience due to its unique advantages,and it also has the potential to transform brain research in non-human primates.Traditional mesoscale connectome techniques typically use fluorescence labeling and optical imaging to perform anterograde or retrograde tracing of neural circuits.To achieve single-cell resolution,methods for sparse labeling of neurons have been developed.However,it remains challenging to trace neurons in high throughput in individual animals and integrate multi-omics data across modalities.In the past decade,high-throughput mesoscale connectome technologies based on DNA barcoding have made significant progress.These technologies have provided novel tools to map single cell connectome,with higher throughput,lower cost,and multi-omics compatibility.Here we review several mature mesoscale connectome technologies based on DNA barcoding,discussing their principles,applications,advantages and disadvantages.We also propose future directions for barcoding-based connectomics.
关键词
DNA条形码/介观联接组/脑联接图谱
Key words
DNA barcode/mesoscale connectome/brain connectivity atlas
维普
万方数据