电子显微成像技术的快速发展使得对完整细胞、组织乃至整个机体进行高分辨三维结构解析研究成为可能，这些可进行大尺度生物样品三维结构研究的电子显微成像技术统称为体电子显微学技术(volume electron microscopy，vEM)。近年来，vEM在研究尺度、分辨率、吞吐量和易用性等方面发展迅速，在整个生命科学领域的应用呈爆炸式增长，该技术因此被《自然》(Nature)评为2023年最值得关注的七项前沿技术之一。然而，vEM相关技术的发展和应用在国内起步较晚，亟待进一步推广。本综述涵盖了 vEM的发展历程、技术分类、样品制备、数据收集、图像处理等全方位的内容，便于生命科学、医学等领域研究人员去了解、学习、应用和进一步发展该技术。
Advances of Volume Electron Microscopy
Volume electron microscopy(vEM)imaging technology was rapidly developed in recent years.It has been the advanced technology to solve high-resolution three-dimensional structures of biological samples.Much wonderful work has revealed the fine structure and interactions of intracellular organelles,the ultrastructure of tissues,and even the three-dimensional structure of entire small biological organisms.With the continuous improvement of resolution,scale and throughput,vEM is becoming more and more widely used in medicine,life sciences,clinical diagnostics and other fields.As a result,this technology has been rated by Nature as one of the seven most noteworthy frontier technologies to watch in 2023.However,the development and application of vEM-related technologies started late in China and need to be further promoted.We write this review to introduce all related vEM technologies,covering the development history of vEM,technology classification,sample preparation,data collection,image processing,etc.,which is convenient for people in various fields to understand,learn,apply and further develop this technology.
volume electron microscopyscanning electron microscopytransmission electron microscopysample preparationimage processingcross-scaledeep learningultrastructure
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维普
