首页|马铃薯StXYLP基因家族的生物信息学分析

马铃薯StXYLP基因家族的生物信息学分析

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  • NETL
  • NSTL
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[目的]在马铃薯基因组水平上鉴定木质形成素类蛋白(Xylogen-like protein，XYLP)基因家族，分析其理化性质及基因的表达模式。[方法]用生信学方法鉴定马铃薯XYLPs，分析其理化性质、亚细胞定位、进化关系、蛋白质结构、基因结构和染色体定位。利用芯片数据分析马铃薯StXYLPs的时空表达模式及对非生物胁迫的响应。[结果]马铃薯中共有20个StXYLPs;蛋白全长在139～221氨基酸之间，理论等电点在4。36～9。42之间，均锚定在质膜上;蛋白质结构基本相似，均具有保守的nsLTP结构域;StXYLPs在染色体上的定位具有偏好性;StXYLPs具有明显差异的组织表达模式，响应不同的非生物胁迫。[结论]StXYLPs与生长素、细胞分裂素和赤霉素共同调控马铃薯器官发育，19个StXY-LPs表达受调控。12个StXYLPs受ABA诱导表达上调，在盐、高温及干旱胁迫下，14个StXYLPs受诱导表达上调，参与对抗非生物胁迫。
Bioinformatics analysis of xylogen-like protein gene family in potato
[Objective]Identification members of the xylogen-like protein(XYLP)gene family in potato at the genome level.Prediction and fully analyzed the physicochemical properties and gene expression patterns of StXYLPs.[Method]Identification and analysis of physicochemical properties,subcellular localization,evolutionary relationship,protein structure,gene structure,and chromosome location of StXYLPs were conducted by bioinformatics methods.Microarray chip was used to analyze the spatio-temporal expression pattern of StXYLPs and the its response to the abiotic stress treatments.[Result]A total of 20 StXYLPs were identified in the potato genome;the full length of the protein was between 139 and 221 amino acids,the theoretical isoe-lectric point was between 4.36 and 9.42,and they were all anchored on the plasma membrane;the structures of StXYLPs are generally similar,and all have the conserved nsLTP domain;StXYLPs have some preference on the chromosome location;ex-pression patterns of StXYLPs are different and genes response much differently to the abiotic stress treatments.[Conclusion]During the organogenesis of potato,expression level of 19 StXYLPs were regulated by hormones and interacted with auxin,cyto-kinin and gibberellin.12 StXYLPs were upregulated by ABA treatment,when plants exposure to the abiotic stresses,such as salt,heat and drought,expression level of 14 StXYLPs were upregulated,thus enhanced plants abiotic stress tolerance.

potatotomatoxylogengene familyxylem developmentabiotic stresshormonebioinformatics

李辰、刘紫涵、桑殊童、陈元爱、徐静、王彩丽

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德州学院生命科学学院,山东德州 253023

马铃薯 番茄 木质形成素 家族基因 木质部发育 非生物胁迫 激素 生物信息学

山东省自然科学基金青年基金山东省自然科学基金青年基金德州学院科学研究基金德州学院科学研究基金德州市科技研发项目

ZR2022QC230ZR2022QC2262021xjrc1052021xjrc2052022dzkj026

2024

10.16519/j.cnki.1004-311x.2024.01.0005

生物技术
黑龙江省微生物学会 黑龙江省生物工程学会 黑龙江省科学院微生物研究所

生物技术

CSTPCD
影响因子：0.611
ISSN：1004-311X
年,卷(期)：2024.34(1)
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