Effect of miRNA-34a-5p targeted CDK6 expression on biological characteristics of lung cancer cells
[Objective]To analyze the effect of miRNA-34a-5p targeted CDK6 expression on the biological characteristics of lung cancer cells and explore the relationship between them.[Method]The expression levels of miRNA-34a-5p,CDK6 mR-NA and CDK6 protein were compared using the surgical tumor tissue samples and paracancerous tissue samples from patients with non-small cell lung cancer treated by surgery in our hospital.The miR-34a-5p inhibitor was transfected into lung cancer A549 cells.To observe its effects on the survival,cloning,apoptosis,migration and invasion of A549 cells.To verify the targeted regulatory relationship of miR-34a-5p on CDK6.[Result]miRNA-34a-5p(0.21±0.05)in tumor tissue was sig-nificantly lower than that in para-cancer tissue(0.54±0.08),while CDK6 mRNA expression level(0.76±0.07)was signifi-cantly higher than that in para-cancer tissue(0.23±0.04)(P＜0.05).The levels of miRNA-34a-5p(0.14±0.03),CDK6 mRNA(0.94±0.12),proliferation inhibition rate(17.32±2.39)％,apoptosis rate(12.32±2.39)％,clone formation number(103.84±7.68),cell migration number(233.21±31.02),cell invasion number(102.16±10.93),CDK-6(0.79± 0.09)and p-AKT(0.88±0.10)in the inhibition plasmid group were higher than those in the blank control group(0.30± 0.05),(0.75±0.09),(29.84±5.01)％(21.23±2.85)％,(77.17±8.43)％,(121.83±20.94)％,(24.39± 5.12)％,(0.45±0.07)％,(0.54±0.08)％and negative control group(0.22±0.07)％,(0.83±0.14)％,(30.17± 4.83)％,(22.08±3.94)％,(74.39±10.95)％,(118.38±26.38)％,(26.14±4.38)％,(0.48±0.06)％,(0.56± 0.07)％(P＜0.05).The fluorescein intensity of Wt-CDK6(0.38±0.09)was significantly lower than that of Mut-CDK6(0.99±0.21),and the expression of CDK-6 protein after miR-34a-5p transfection(0.28±0.05)was lower than that of the negative control(0.95±0.11),the difference was statistically significant(P＜0.05).[Conclusion]miRNA-34a-5p can directly target the expression of CDK6 to regulate the biological characteristics of lung cancer cells,such as proliferation,apopto-sis,invasion,metastasis,and clonogenesis.
NETL
NSTL
万方数据
