Research progress of protein secretion mediated by α-mating factor in Pichia pastoris
The Pichia pastoris expression system is widely used for the secretion and expression of heterologous proteins.How to improve the expression levels of heterologous proteins is the focus of the research in the system.The efficiency of secreted proteins through the secretory pathway is a limiting factor for heterologous protein production.The α-mating factor signal lead-er is commonly used in Pichia pastoris and the α-mating factor signal leader from Saccharomyces cerevisiae,which can mediate the secretion and expression of most proteins in P.pastoris and alpha-mating factor of other species guided protein expression in Pichia pastoris.Based on the important role of S.cerevisiae α-mating factor secretion signal in the production of recombinant proteins in P.pastoris expression system,in this paper,we systematically review its research progress on protein secretion in P.pastoris.The structural and functional characteristics of α-mating factor are described and the analysis are put forward.The fu-ture research of α-mating factor is prospected.
NETL
NSTL
万方数据
