Proteome response of Gram-negative bacteria in subinhibitory environment
[Objective]To study the antimicrobial resistance of microorganisms in the subinhibitory environment,and to ex-plore the proteomic response of microorganisms in the subinhibitory environment.[Method]Two common gram-negative bacte-ria,namely Escherichia coli and Salmonella sensitive strains,were induced by ciprofloxacin and enrofloxacin into drug-resistant strains in a subinhibitory environment,and whole bacterial proteins were extracted.The differentially expressed proteins were analyzed by Label-free quantitative proteomics combined with liquid chromatography-mass spectrometry(LC-MS).[Re-sult]When Gram-negative bacteria were passed under subinhibitory environment,the minimum inhibitory concentration was increased to 10 μg/L,indicating that drug resistance was developed.Proteomic difference analysis showed that more than 60％of the drug-resistance differential proteins of E.coli and Salmonella were enriched in cell membrane components,mainly in biofilm formation,effector pump formation,amino acid metabolism,TCA cycle and other pathways related to energy metabolism and nucleic acid metabolism.[Conclusion]Gram negative bacteria can still develop drug resistance in the subinhibitory environ-ment.Proteomic difference analysis results show that the bactericidal action of drugs can be resisted by adjusting the pathways related to energy metabolism and nucleic acid metabolism,so as to adjust the drug resistance status,which lays a theoretical foundation for further study of the physiological and biochemical changes of microorganisms in the subinhibitory environment.
Escherichia coliSalmonellaciprofloxacinenrofloxacinsuinhibitory drug environmentdrug resistancepro-teomicsresponse law
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