Production of 4-androstenedione by biotransforming egg yolk cholesterol
[Objective]To explored a method of using egg yolk as the raw material,and biotransforming 4-androstenedione(AD)by HGMS6 strain,achieving a conversion rate of 60.78％.[Method]Based on the existing extraction and transformation technologies,the extraction conditions of egg yolk cholesterol and the fermentation environment of actinomycetes were adjusted,and the data were analyzed by thin-layer chromatography and high-performance liquid chromatography to improve the yield of AD.[Result]The optimal extraction conditions of egg yolk cholesterol were determined as follows:β-CD∶cholesterol=5∶1,water:β-CD=15∶1,reaction temperature was 55 ℃.The extraction rate was 98.12％.Compared with Tween-80,adding cy-clodextrin resulted in a higher cholesterol conversion rate(60.18％).
egg yolkcholesterolmicrobial transformationsteroid drug intermediate4-androstenedioneactinomycetes
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