Puerarin affects the relaxation and contraction of detrusor muscle in rats by regulating Wnt/β-catenin pathway
[Objective]To analyze the effect of puerarin on the detrusor contraction and Wnt/β-catenin pathway in animal models.[Method]Fifty rats were randomly divided into a control group,a model group,a puerarin 10 mg group,a puerarin 20 mg group,and a puerarin 30 mg group;the model group and the puerarin group rats established a model of suprasacral spi-nal cord injury After the intervention of puerarin and saline according to grouping;one month later,the urodynamics of rats in each group were detected,and the levels of α1G,c-kit,Wnt1 and β-catenin in the tissue were analyzed.[Result]There were significant differences in the protein and mRNA levels of MBC,BLPP,CF,α1G,c-kit,Wnt1 and β-catenin in the control group,model group,puerarin 10 mg group,puerarin 20 mg group and puerarin 30 mg group(P＜0.05),and the protein and mRNA levels of MBC,BLPP,CFF,α1G,c-kit,Wnt1 and β-catenin in the puerarin group were significantly different from the model group(P＜0.05),among the puerarin groups,the puerarin 30 mg group had the highest protein and mRNA levels of MBC,Wnt1 and β-catenin,and the lowest protein and mRNA levels of BLPP,CF,α1G and c-kit(P＜0.05).[Conclusion]Puerarin can effectively activate Wnt/β-catenin pathway and improve detrusor muscle contraction and contraction in animal models.
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