[目的]探究DKK1与PI3K/AKT信号通路在胃癌细胞的EMT进程中的作用。[方法]免疫组化实验分析胃癌组织以及癌旁正常组织中DKK1的表达水平;将胃癌细胞系MGC-803细胞分为3组:si DKK1组、si NC组和LY294002组。MTT实验分析MGC-803细胞的增殖能力;Transwell实验分析细胞的侵袭能力;细胞划痕实验分析细胞的迁移能力;蛋白免疫印迹实验分析PI3K/AKT信号通路蛋白表达的情况。[结果]与胃癌的癌旁正常组织比较，胃癌组织DKK1的表达水平升高。与si NC组比较，si DKK1和LY294002组的MGC-803细胞生长速度显著下降(2。89±0。02 vs 1。59±0。05 vs 1。37±0。03，P＜0。05);si DKK1 和 LY294002 组的 MGC-803 细胞侵袭数量显著降低(132。23±10。02 vs 71。39±7。05 vs 68。57±8。03，P＜0。05);si DKK1 和 LY294002 组的 MGC-803 细胞迁移比例显著降低(0。83±0。03 as 0。39±0。01 vs 0。41±0。03，P＜0。05);si DKK1 和 LY294002 组的 MGC-803 细胞 PI3K/AKT 蛋白磷酸化水平降低(P＜0。05)。[结论]DKK1在胃癌组织中高表达，下调DKK1表达后，胃癌MGC-803细胞的增殖、侵袭和迁移能力受到显著抑制，这一过程与下调DKK1抑制PI3K/AKT信号通路激活密切相关。
DKK1 regulates the PI3K/AKT pathway to affect the EMT process of gastric cancer cells
[Objective]To investigate the role of DKK1 and PI3K/AKT signaling pathway in the EMT process of gastric cancer cells.[Method]The expression level of DKK1 in gastric cancer tissues and adjacent normal tissues was analyzed by immuno-histochemistry.Gastric cancer cell line MGC-803 cells were divided into three groups:si DKK1 group,si NC group and LY294002 group.The proliferation ability of MGC-803 cells was analyzed by MTT assay.Transwell assay was used to analyze cell invasion ability.The migration ability of cells was analyzed by cell scratch test.The protein expression of PI3K/AKT signa-ling pathway was analyzed by Western Blot.[Result]Compared with the adjacent normal tissues,the expression level of DKK1 in gastric cancer tissues was increased.Compared with the si NC group,the growth rate of MGC-803 cells in the si DKK1 and LY294002 groups was significantly decreased(2.89±0.02 vs 1.59±0.05 vs 1.37±0.03,P＜0.05).The number of invasive MGC-803 cells in the si DKK1 and LY294002 groups was significantly decreased(132.23±10.02 vs 71.39±7.05 vs 68.57±8.03,P＜0.05).The migration rate of MGC-803 cells in the si DKK1 and LY294002 groups was significantly decreased(0.83±0.03 vs 0.39±0.01 vs 0.41±0.03,P＜0.05).The phosphorylation level of PI3K/AKT protein in MGC-803 cells was decreased in the si DKK1 and LY294002 groups(P＜0.05).[Conclusion]DKK1 is highly expressed in gastric cancer tissues,and the proliferation,invasion and migration of MGC-803 cells are significantly inhibited by DKK1 knockdown,which is closely related to the inhibition of PI3K/AKT signaling pathway activation by DKK1.
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