Preparation and identification of monoclonal antibodies against diacetylspermine(DAS)
[Objective]To prepare diacetylspermine(DAS)monoclonal antibodies by hybridoma technique,and to character-ize them and study their applications.[Method]Diacetylspermine(DAS)immunogen was prepared and immunized in Balb/c healthy female mice,and the mouse spleen cells were fused with SP2/0 myeloma cells.The diacetylspermine(DAS)antibody was purified,specific antibody identification and clinical application.[Result]The specific monoclonal antibody was obtained,the purity of DAS monoclonal antibody was qualified and the stability was good,and the DAS immunoturbidimetric kit was made.The detection rate of diacetylspermine(DAS)in 184 cases of colorectal cancer was 65.22％.The detection rate of CEA in 170 cases of colorectal cancer was 40.00％.The detection rate of AFP in 93 cases of colorectal cancer was 2.15％.The de-tection rate of CA199 in 166 cases of colorectal cancer was 17.47％.The detection rate of CA724 in 30 cases of colorectal cancer was 50％.The detection rate of CA125 in 87 cases of colorectal cancer was 14.94％.[Conclusion]Diacetylspermine(DAS)monoclonal antibody with good specificity and stability was successfully prepared and can be used for the early clinical diagnosis of intestinal,hepatocellular and gastric cancers.
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