Functional analysis of CrLEC1 gene regulating lipid synthesis in Chlamydomonas reinhardtii
[Objective]To provide a basis for establishing breeding strategies for cultivating high oil content and high biomass microalgae through genetic engineering,the regulatory function of the transcription factor LEC1(Leaf cotyledon 1)in microalgae oil biosynthesis will be analyzed.[Method]An overexpression vector containing CrLEC1 gene were constructed by seamless cloning method,Chlamydomonas reinhardtii CC849 cells were transformed by glass bead transformation method,and the oil con-tent of transgenic algae strains were determined by Nile red fluorescence assay.[Result]The plasmidpCam1304::LEC1::GFP were successfully constructed.137 positive transformed algae strains were obtained,of which 16 transgenic algae strains were detected.The growth of transgenic algae strains 6-15 was not affected by nitrogen stress,and their growth status was better than that of wild-type algae strains under nitrogen deficient or nitrogen containing culture conditions.Under nitrogen deficient conditions,the oil content of transgenic algae strains 6-15,6-9,5-22,5-25 and 5-34 was significantly higher than that of wild-type algae strains.[Conclusion]CrLEC1 gene was positively regulating lipid synthesis in Chlamydomonas reinhardtii.
NETL
NSTL
万方数据
