Immune-associated ceRNA in the development and metastasis of early-onset colorectal cancer
[Objective]To investigate immune-associated competitive endogenous RNA(ceRNA)in the development and metastasis of early-onset colorectal cancer(EOCRC).[Method]The RNA-seq data of EOCRC was downloaded from the TCGA database,and the immune-related ceRNA networks for tumor occurrence,metastasis and prognosis were constructed after differential expression analysis,IMMPORT online tool,Kaplan-Meier survival curve and functional enrichment analysis,respectively.Therefore,hub genes and pathways were obtained.[Result]There were 127 lncRNAs,35 miRNAs and 25 immune-related mRNAs differentially expressed in 92 cases of EOCRC between tumor tissues and normal tissues.MOCODE1 played an important role in constructing the immune-related ceRNA network of tumorigenesis,which was mainly concentrated in MAPK signaling pathway.Survival analysis was used to construct prognostic and metastatic ceRNA networks respectively.It was found that HCFC1-AS1-hsa-miR-145-5p-PDGFD was a co-regulation axis and a major component of MOCODE1.The expression of this axis changed from low to high during tumorigenicity to metastasis.[Conclusion]The HCFC1-AS1-hsa-miR-145-5p-PDGFD axis was the hub ceRNA in the occurrence of EOCRC.It's down-regulated expression promoted tumorigenesis through MAPK signaling pathway.Upregulation of HCFC1-AS1 and hsa-miR-145-5p was associated with metastasis(P=3.711e-02 & 2.682e-02)and poor prognosis(P=2.578e-02 & 1.233e-02),and PDGFD was a poten-tial drug therapeutic target.
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