TE fusion gene promotes the metastasis of prostate cancer cells by regulating Wnt/β-catenin pathway
[Objective]To investigate the effect of TE fusion gene on the metastasis of prostate cancer DU145 cells.[Method]DU145 cells were randomly divided into three experimental groups:siRNA NC group,siRNA T2E group and KYA1797K group.The proliferation of DU145 cells was detected by MTT assay.Transwell assay was used to detect the invasion ability of DU145 cells.The apoptosis rate of DU145 cells was detected by flow cytometry.The protein expression of Wnt/β-catenin pathway in DU145 cells was analyzed by Western Blot.[Result]Compared with the siRNA NC group,the proliferation ability of DU145 cells in the siRNA T2E and KYA1797K groups decreased(1.73±0.35 vs 1.02±0.22 vs 0.91±0.27,P＜0.05).The inva-sion number of DU145 cells in siRNA T2E and KYA1797K groups decreased(167.28±12.89 vs 78.21±9.26 vs 82.33±10.33,P＜0.05).The apoptosis rate of DU145 cells in siRNA T2E and KYA1797K groups was significantly increased(8.25％±0.89％vs 28.82％±2.56％vs 29.46％±1.83％,P＜0.05).The protein expressions of Wnt3α and β-catenin in DU145 cells in siRNA T2E group and KYA1797K group were down-regulated(P＜0.05).[Conclusion]After inhibiting the expression of TE fusion gene,the proliferation rate and invasion number of DU 145 cells was decreased,and the apoptosis rate is increased,which is related to the regulation of Wnt/β-catenin pathway by TE fusion gene.
TE fusion geneWntmetastasisβ-cateninprostate cancerapoptosisDU145siRNA
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