Effect of miR-143-3p on the metastatic activity of triple-negative breast cancer cells in vitro by targeting HMGA2
[Objective]To investigate the effect of miR-143-3p and HMGA2 on the biological process of triple-negative breast cancer cells.[Method]The expression levels of miR-143-3p in different tissues were compared by real-time fluores-cence quantitative PCR,and the expression of HMGA2 in different tissues was detected by immunohistochemistry.The MDA-MB-231 cell line was divided into four groups:miR NC group,miR-143-3pmimic group,inhibitor NC group and HMGA2 inhibitor group.Protein immunoblotting was used to detect the protein expression level of HMGA2;cell colony formation assay was used to study the growth ability of cells;Transwell assay was used to study the invasion ability of cells;cell scoring assay was used to analyze the migration ability of cells;flow cytometry was used to detect the apoptosis rate;bioinformatics method was applied to predict the binding site of miR-143-3p and HMGA2;dual luciferase reporter gene assay was performed to an-alyze the targeting relationship between miR-143-3p and HMGA2.[Result]The expression level of miR-143-3p was de-creased(0.98±0.02 vs 0.46±0.03)and HMGA2 was increased(P＜0.05)in triple-negative breast cancer tissues.miR-143-3p could inhibit the activity of HMGA2(1.09±0.02 vs 0.32±0.01,P＜0.05).miR-143-3p mimic or HMGA2 in-hibitor could inhibit the growth,migration and invasion of MDA-MB-231 cells,the apoptosis of MDA-MB-231 cells(4.69％±0.02％vs 16.32％±1.01％vs 4.17％±0.05％vs 15.22％±0.03％;P＜0.05).[Conclusion]HMGA2 is a direct target of miR-143-3p.miR-143-3p may inhibit the metastatic activity of triple-negative breast cancer cells in vitro by targeting and regulating HMGA2,and the relationship between miR-143-3p and HMGA2 is expected to be a key target for the treatment of triple-negative breast cancer.
miR-143-3ptriple-negative breast cancerHMGA2metastatic activityinvasionmigrationproliferationMDA-MB-231
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