[目的]探究薯蓣皂苷调节Wnt/β-catenin通路对口腔鳞癌HSC4细胞的体外转移能力的影响。[方法]将口腔鳞癌HSC4细胞株随机分为3组:空白对照组、薯蓣皂苷组以及KYA1797K组。蛋白免疫印迹实验检测Wnt/β-cate-nin 通路相关蛋白的表达水平;采用MTT实验分析HSC4细胞的增殖能力;Transwell法分析HSC4细胞的侵袭能力;细胞划痕实验分析HSC4细胞迁移能力;TUNEL实验分析HSC4细胞凋亡率。[结果]MTT实验和Transwell实验结果显示，薯蓣皂苷或KYA1797K均能抑制口腔鳞癌HSC4细胞的生长与侵袭能力(79。57±3。78 vs 38。21±1。89 vs 32。19±2。18个，P＜0。05);细胞划痕实验结果显示，薯蓣皂苷或KYA1797K均能抑制口腔鳞癌HSC4细胞的迁移能力(0。69±0。08 vs 0。36±0。09 vs 0。31±0。08，P＜0。05);流式细胞术实验结果显示，薯蓣皂苷或KYA1797K均能促进口腔鳞癌HSC4细胞凋亡(0。31±0。01 vs 0。59±0。02 vs 0。56±0。03，P＜0。05);蛋白免疫印迹实验结果显示，薯蓣皂苷或KYA1797K 均能抑制 Wnt/β-catenin 通路相关蛋白的表达水平(0。87±0。07 vs 0。46±0。06 vsO。51±0。08;0。71±0。06 is 0。37±0。05 vs0。33±0。06;P＜0。05)o[结论]薯蓣皂苷可以明显抑制口腔鳞癌HSC4细胞的增殖、迁移和侵袭能力，并能促进口腔鳞癌HSC4细胞凋亡。薯蓣皂苷的这一作用与抑制Wnt/β-catenin信号通路相关。
Effect of dioscin on the metastatic activity of oral squamous cell carcinoma cells by regulating Wnt/β-catenin pathway
[Objective]To investigate the effect of dioscin on the metastasis of oral squamous cell carcinoma HSC4 cells in vitro by regulating Wnt/[3-catenin pathway.[Method]Oral squamous cell carcinoma HSC4 cells were randomly divided into three groups:control group,dioscin group and KYA1797K group.Western Blot was used to detect the expression levels of Wnt/β-catenin pathway-related proteins.MTT assay was used to analyze the cell proliferation ability.The migration ability of HSC4 cells was analyzed by wound healing assay.Transwell assay was used to analyze cell invasion ability.TUNEL assay was used to analyze the apoptosis rate.[Result]MTT assay and Transwell assay showed that both dioscin and KYA1797K inhibited the growth and invasion of HSC4 cells(79.57±3.78 vs 38.21±1.89 vs 32.19±2.18,P＜0.05).Wound healing assay showed that both dioscin and KYA1797K inhibited the migration of HSC4 cells(0.69±0.08 vs 0.36±0.09 vs 0.31±0.08,P＜0.05).The results of flow cytometry showed that dioscin or KYA1797K could promote the apoptosis of HSC4 cells(0.31±0.01 vs 0.59±0.02 vs 0.56±0.03,P＜0.05).Western Blot results showed that both dioscin and KYA1797K could inhibit the expression of Wnt/β-catenin pathway related proteins(0.87±0.07 vs 0.46±0.06 vs 0.51±0.08;0.71±0.06 vs 0.37±0.05 vs 0.33±0.06;P＜0.05).[Conclusion]Dioscin significantly inhibited the proliferation and invasion of HSC4 cells,and promoted the apoptosis of HSC4 cells.This effect of dioscin is related to the inhibition of Wnt/β-catenin signaling path-way.
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