蜘蛛果多糖对RAW264.7免疫调节作用
Immunomodulatory effect of Campanumoeae lancifoliorum Radix polysaccharides on RAW264.7 cells
代道蝶 1刘梦鸽 1孙庆文 2徐文芬 2陈亮 2万晓霞 1陈春伶2
作者信息
- 1. 贵州中医药大学药学院,贵阳贵州 550025
- 2. 贵州中医药大学药学院,贵阳贵州 550025;贵州中医药大学红果参资源与开发研究中心,贵阳贵州 550025
- 折叠
摘要
[目的]从轮钟花的根中提取出蜘蛛果多糖(Spider fruit polysaccharide)并研究其对RAW264.7细胞的免疫调控作用.[方法]通过超声提取法提取蜘蛛果多糖,CCK-8法检测(脂多糖)LPS对RAW264.7细胞增殖的情况,硝酸还原酶法测定蜘蛛果多糖对RAW264.7细胞代谢NO的水平,ELISA法测定蜘蛛果多糖对RAW264.7细胞分泌TNF-α、1L-6水平的情况.[结果]蜘蛛果多糖在25 μg/mL～400 μg/mL浓度范围内能促进RAW264.7细胞的增殖;与空白对照组相比,蜘蛛果多糖能显著增加RAW264.7细胞的NO、IL-6及TNF-α分泌量,与LPS模型组相比,则能显著降低RAW264.7细胞的NO、TL-6及TNF-α分泌量.[结论]蜘蛛果多糖可以激活巨噬细胞,调节机体免疫力.
Abstract
[Objective]To study the immunoregulatory activity of Campanumoeae lancifoliorum Radix polysaccharides on RAW264.7 cells.[Method]Campanumoeae lancifoliorum Radix polysaccharides were extracted by ultrasonic extraction.The proliferation of RAW264.7 cells was detected by CCK-8 method.The effect of Campanumoeae Lancifoliorum Radix polysac-charides on the secretion of NO in RAW264.7 cells was determined by nitrate reductase method.The effect of Campanumoeae lancifoliorum Radix polysaccharides on the secretion of TNF-α and IL-6 in RAW264.7 cells was determined by ELISA method.[Result]Campanumoeae lancifoliorum Radix polysaccharides could promote the proliferation of RAW264.7 cells in the concentration range of 25 μg/mL-400 μg/mL,and significantly reduce the secretion of NO,IL-6 and TNF-α by RAW264.7 cells induced by LPS.[Conclusion]Campanumoeae lancifoliorum Radix polysaccharides has a certain regulatory effect on the immune system.
关键词
蜘蛛果/轮钟花/多糖/免疫调节/细胞因子/RAW264.7Key words
Campanumoeae lancifoliorum Radix/Cyclocodon lancifolius/polysaccharide/immune regulation/cytokines/RAW264.7引用本文复制引用
基金项目
贵州省科技计划项目(黔科合支撑[2021]一般477)
贵州省科技计划项目(黔科合基础-ZK[2022]一般458)
2023年贵州省财政种业发展项目()
出版年
2024
NETL
NSTL
万方数据